【研究成果】2011

研究成果23

Dynamic SERS Imaging of Cellular Transport Pathways with Endocytosed Gold Nanoparticles
Jun Ando, Katsumasa Fujita, Nicholas I. Smith, and Satoshi Kawata
Nano Lett 2011, 11, 5344-5348

Dynamic SERS imaging inside a living cell is demonstrated with the use of a gold nanoparticle, which travels through the intracellular space to probe local molecular information over time. Simultaneous tracking of particle motion and SERS spectroscopy allows us to detect intracellular molecules at 65 nm spatial resolution and 50 ms temporal resolution, providing molecular maps of organelle transport and lisosomal accumulation. Multiplex spectral and trajectory imaging will enable imaging of specific dynamic biological functions such as membrane protein diffusion, nuclear entry, and rearrangement of cellular cytoskeleton.