【研究成果】2013年
研究成果60
Simultaneous imaging of protonated and deprotonated carbonylcyanide p-trifluoromethoxyphenylhydrazone in live cells by Raman microscopy
Hiroyuki Yamakoshi, Almar F. Palonpon, Kosuke Dodo, Jun Ando, Satoshi Kawata, Katsumasa Fujita, and Mikiko Sodeoka
Chem. Commun., Accepted Manuscript (2013)
We report the simultaneous imaging of protonated and deprotonated forms of carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP) molecules in live cells by Raman microscopy. Nitrile, a structure-sensitive Raman tag, is used to detect the two distinct molecular structures, demonstrating the potential of Raman microscopy for structure-based imaging of bioactive small molecules.
Raman imaging of protonated and deprotonated forms of FCCP in live HeLa cells. Raman images were obtained from HeLa cells treated with 100 uM FCCP. The signals at 2197 and 2230 cm-1 were assigned to the red and green channels, respectively, and a merged image was generated. The laser wavelength was 532 nm. The light intensity at the sample plane was 4.2 mW/um2. The exposure time for each line was 10 sec. The total number of lines was 140.
