【研究成果】2016年-
研究成果103
Formation of fluorescent platinum nanoclusters using hyper-branched polyethylenimine and their conjugation to antibodies for bio-imaging
Xin Huang, Hidekazu Ishitobi & Yasushi Inouye
RSC Advances 6, 9709-9716 (2016) | DOI: 10.1039/c5ra24522b
The formation of yellow fluorescent polyethylenimine-protected platinum nano-clusters (Pt NCs@PEI) synthesized by a facile one-pot reduction method was investigated. Pt NCs were produced in the cavities formed by coiled PEI ligands and were mostly stabilized with the amino groups. Pt NCs@PEI with an average diameter of 1.4 ± 0.4 nm demonstrated excellent photo-stability against high salt concentration and long-term light exposure. By conjugating Pt NCs@PEI with the chemokine receptor anti-CXCR4 antibody, Pt NCs@PEI-(anti-CXCR4-Ab) conjugates were successfully applied to bio-imaging of the membrane of live HeLa cells that had their nuclei stained with DAPI. Moreover, Pt NCs@PEI showed lower cell cytotoxicity than Qdots@COOH, indicating that they have better cell viability and great potential for bio-applications.
(a) TEM micrograph of Pt NCs@PEI (scale bar=10 nm)
(b) histograms of their size-distribution.
(a) differential interference contrast imaging &
(b) laser confocal fluorescence microscopy of living HeLa cells labelled with Pt NCs@PEI -(anti-CXCR4-Ab) conjugates (scale bar=20 µm)
