【研究成果】2015年

研究成果86

Nonlinear fluorescence imaging by photoinduced charge separation
Kentaro Mochizuki, Lanting Shi, Shin Mizukami, Masahito Yamanaka, Mamoru Tanabe, Wei-Tao Gong, Almar F Palonpon, Shogo Kawano, Satoshi Kawata, Kazuya Kikuchi, and Katsumasa Fujita
Japanese Journal of Applied Physics 54, 042403 (2015)

For super-resolution microscopy, the authors developed a new strategy to realize a probe with a nonlinear fluorescence response, a stepwise two-photon absorption scheme, by using photoinduced charge separation; the first photon for the generation of the charge-separated state and the second photon for fluorescence excitation. Transient absorption spectra studies and simulation indicate that fluorescence is emitted through the photophysical pathways they proposed. Fluorescence imaging of biological cells showed marked improvements in image contrast and resolution, demonstrating the usefulness of the fluorescent probe in laser scanning confocal microscopy.

Fluorescence images of HeLa cells stained with (a) nitro-bisBODIPY and (b) BODIPY, measured with a laser scanning confocal microscope. The scales for signal values are the same in both images.