【研究成果】2012

研究成果56

No-wash protein labeling with designed fluorogenic probes and application to real-time pulse-chase analysis
Mizukami S, Watanabe S, Akimoto Y, Kikuchi K.
J Am Chem Soc. 2012 Jan 25;134(3):1623-9. Epub 2012 Jan 6.

A no-wash fluorogenic labeling system by exploiting fluorescence resonance energy transfer (FRET)-based fluorescein-cephalosporin-azopyridinium probes and a mutant β-lactamase tag. Fast quencher elimination, hydrophilicity, and high resistance against autodegradation were achieved by rational refinement of the structure. By applying the probe to real-time pulse-chase analysis, the trafficking of epidermal growth factor receptors between cell surface and intracellular region was imaged. In addition, membrane-permeable derivatization of the probe enabled no-wash fluorogenic labeling of intracellular proteins.